Immunological Sciences

Phospho-Crk II (Y221)

Western blot analysis of extracts of K562 cells, using Phospho-CRK-Y221 antibody at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour . Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 10s.

Western blot analysis of extracts of K562 cells, using Phospho-CRK-Y221 antibody at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour . Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:ABP10284
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A synthetic phosphorylated peptide around Y221 of human CRK.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500-1:2000
Molecular Weight:38kDa
Purification:Affinity Purification
Form:liquid
Synonyms:CRK;CRKII;p38

Applications

Western Blot: 1:500-1:2000

Immunogen

A synthetic phosphorylated peptide around Y221 of human CRK.

Target Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2- phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described

Synonyms:CRK;CRKII;p38

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of K562 cells, using Phospho-CRK-Y221 antibody at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour . Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 10s.

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