Phospho-Crk-L (Y207)

Western blot analysis of extracts of K-562 cells, using Phospho-CRKLY207 antibody at 1:1000 dilution or CRKL antibody.         K-562 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 5s.

Western blot analysis of extracts of K-562 cells, using Phospho-CRKLY207 antibody at 1:1000 dilution or CRKL antibody. K-562 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 5s.

Catalog Number:ABP10285
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A synthetic phosphorylated peptide around Y207 of human CRKL.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100WB, IHC
Molecular Weight:38kDa
Purification:Affinity Purification
Form:liquid

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100WB, IHC

Immunogen

A synthetic phosphorylated peptide around Y207 of human CRKL.

Target Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of K-562 cells, using Phospho-CRKLY207 antibody at 1:1000 dilution or CRKL antibody. K-562 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 5s.

Immunohistochemistry of paraffin embedded rat brain using Phospho- CRKL-Y207 antibody at dilution of 1:100 (40x lens).

No documents available.