Immunological Sciences

Phospho-eIF2α (S51) Rabbit Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 Rabbit MAb at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 Rabbit MAb at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Catalog Number:MAB-94689
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:D9G8
Immunogen:A synthetic phosphorylated peptide around S51 of human eIF2a
Reactivity:Hu, Ms, Rt
Applications:WB 1:500 - 1:2000
Molecular Weight:38 kDa
Purification:Affinity purification
Form:liquid
Synonyms:EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A;EIF2S1

Applications

WB 1:500 - 1:2000

Immunogen

A synthetic phosphorylated peptide around S51 of human eIF2a

Target Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Synonyms:EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A;EIF2S1

Storage

Store at –20°C. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3

Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 Rabbit MAb at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

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