Immunological Sciences

Phospho-EIF4EBP1 (T37/46)

Western blot analysis on Hela cells using Phospho-EIF4E-BP1 (Thr37/46) Antibody

Western blot analysis on Hela cells using Phospho-EIF4E-BP1 (Thr37/46) Antibody

Catalog Number:ABP-0030
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Reactivity:Hu, Ms, Rt
Applications:WB: 1:1000
Molecular Weight:15-20 kDa
Purification:Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 of mouse EIF4EBP1 and Thr46 of mouse EIF4E-BP1. Antibodies are purified by protein A and peptide affinity chromatography
Form:liquid

Applications

WB: 1:1000

Target Background

Translation repressor protein EIF4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of EIF4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate EIF4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of EIF4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). Phospho-EIF4E-BP1 (Thr37/46) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites

Storage

Store at –20°C. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis on Hela cells using Phospho-EIF4E-BP1 (Thr37/46) Antibody

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