Immunological Sciences

Phospho-EIF4EBP1 (T37/46)

Western blot analysis on Hela cells using Phospho-4E-BP1 (Thr37/46) (236B4) monoclonal antibody

Western blot analysis on Hela cells using Phospho-4E-BP1 (Thr37/46) (236B4) monoclonal antibody

Catalog Number:MAB-94214
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:236B4
Reactivity:Hu, Ms, Rt
Applications:WB 1:1000
Molecular Weight:15-20 kDa
Purification:: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1 protein.
Form:liquid

Applications

WB 1:1000

Target Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence.

Storage

Store at –20°C. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis on Hela cells using Phospho-4E-BP1 (Thr37/46) (236B4) monoclonal antibody

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