Phospho-eNOS-S1177

Western blot analysis of extracts of SKOV3 cells, using Phospho-eNOS S1177 antibody SKOV3cells were treated by EGF. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.  Blocking buffer: 3% BSA.

Western blot analysis of extracts of SKOV3 cells, using Phospho-eNOS S1177 antibody SKOV3cells were treated by EGF. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Catalog Number:ABP-0515
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A synthetic phosphorylated peptide around S1177 of human eNOS.
Reactivity:Hu, Ms
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200
Molecular Weight:133 kDa
Purification:Affinity purification
Form:liquid
Synonyms:NOS3;ECNOS;Enos

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200

Immunogen

A synthetic phosphorylated peptide around S1177 of human eNOS.

Target Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Synonyms:NOS3;ECNOS;Enos

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of SKOV3 cells, using Phospho-eNOS S1177 antibody SKOV3cells were treated by EGF. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-eNOSS1177 antibody at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 60s.

Immunohistochemistry of paraffin embedded mouse pancreas using Phospho-NOS3-S1177 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin embedded mouse spinal cord using Phospho-NOS3-S1177 antibody at dilution of 1:200 (40x lens).

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