Immunological Sciences

Phospho-ERK 1/2 (Thr202/Y204) Rabbit Polyclonal Antibody

Western Blot analysis of various cells using Phospho-ERK 1/2 (T202/Y204) Polyclonal Antibody

Western Blot analysis of various cells using Phospho-ERK 1/2 (T202/Y204) Polyclonal Antibody

Catalog Number:ABP11212
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Synthesized phospho- peptide around the phosphorylation site of human ERK 1/2 (phospho Thr202/Y204)
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1: 500 – 1:2000Immunofluorescence: 1:50-1:200Immunohistochemistry: 1:100 – 1:300ELISA: 1:20000
Molecular Weight:44+42 kDa
Purification:The antibody was affinity- purified from rabbit antiserum by affinity- chromatography using epitope- specific immunogen.
Form:Liquid
Synonyms:MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK- 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44- MAPK; Microtubule-associated protein 2 kinase;
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Applications

Western Blot: 1: 500 – 1:2000Immunofluorescence: 1:50-1:200Immunohistochemistry: 1:100 – 1:300ELISA: 1:20000

Immunogen

Synthesized phospho- peptide around the phosphorylation site of human ERK 1/2 (phospho Thr202/Y204)

Target Background

mitogen-activated protein kinase 3(MAPK3) Homo sapiens The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal- regulated kinases ( ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Synonyms:MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK- 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44- MAPK; Microtubule-associated protein 2 kinase;

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using Phospho-ERK 1/2 (T202/Y204) Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, ERK 1/2 (phospho Thr202/Y204) Polyclonal Antibody was diluted at 1:200 (4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of various cells using Phospho-ERK 1/2 (T202/Y204) Polyclonal Antibody

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