Immunological Sciences

Phospho-ERK1-T202/Y204 + ERK2-T185/Y187

Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2- T185/Y187 antibody at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico plus. Exposure time: 180s.

Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2- T185/Y187 antibody at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico plus. Exposure time: 180s.

Catalog Number:ABP-0472
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A synthetic phosphorylated peptide around T185/Y187 of human ERK2.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100
Molecular Weight:42KDa/44KDa
Purification:Affinity purification
Form:liquid
Synonyms:MAPK1/MAPK3

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100

Immunogen

A synthetic phosphorylated peptide around T185/Y187 of human ERK2.

Target Background

This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]

Synonyms:MAPK1/MAPK3

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2- T185/Y187 antibody at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico plus. Exposure time: 180s.

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb. Western blot was performed from the immunoprecipitate using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb at a dilition of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.

Immunohistochemistry of paraffin embedded human colon using Phospho- ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded rat testis using Phospho-ERK1- T202/Y204 + ERK2-T185/Y187 Rabbit pAb at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded mouse spinal cord using Phospho-ERK1-T202/Y204 + ERK2- T185/Y187 Rabbit pAb at dilution of 1:100 (40x lens).

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