Immunological Sciences

Phospho-Ezrin (T566)

Western Blot (WB) analysis of HeLa COLO205 using Phospho-Ezrin (T566) antibody

Western Blot (WB) analysis of HeLa COLO205 using Phospho-Ezrin (T566) antibody

Catalog Number:ABP-10001
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:Synthesized peptide derived from human Ezrin around the phosphorylation site of T566.
Reactivity:Hu, Ms, Rt
Applications:Western Blot 1:1,000 ELISA: 1:10,000
Molecular Weight:69 kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity- chromatography using epitope-specific immunogen.
Form:liquid

Applications

Western Blot 1:1,000 ELISA: 1:10,000

Immunogen

Synthesized peptide derived from human Ezrin around the phosphorylation site of T566.

Target Background

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.Phospho-Ezrin (T566) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at T566

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot (WB) analysis of HeLa COLO205 using Phospho-Ezrin (T566) antibody

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