Phospho-IGF-1R (Y1161)

Western Blot (WB) analysis of specific cells using Phospho-IGF-IR (Y1161) Polyclonal Antibody.

Western Blot (WB) analysis of specific cells using Phospho-IGF-IR (Y1161) Polyclonal Antibody.

Catalog Number:ABP-0367
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:Synthesized peptide derived from human IGF-IR around the phosphorylation site of Y1161. Immunogen Region: 1100-1180 aa
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:1000 Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:40000
Molecular Weight:95 kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.
Form:liquid

Applications

Western Blot: 1:1000 Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:40000

Immunogen

Synthesized peptide derived from human IGF-IR around the phosphorylation site of Y1161. Immunogen Region: 1100-1180 aa

Target Background

Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.Specificity: Phospho-IGF-IR (Y1161) Polyclonal Antibody detects endogenous levels of IGF-IR protein only when phosphorylated at Y1161.

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot (WB) analysis of specific cells using Phospho-IGF-IR (Y1161) Polyclonal Antibody.

mmunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for anbitody retrieval. Negetive contrl (right) obtaned from antibody was pre-abso

Immunofluorescence staining - Phospho-IGF1R-Y1161 antibody

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