Immunological Sciences

Phospho-IκB-α (S32/S36) Rabbit Polyclonal Antibody

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with TNF-alpha for 5 minutes., using Phospho-IkB-alpha (Ser32) Rabbit  (upper), or IkB-alpha Rabbit  (lower).

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with TNF-alpha for 5 minutes., using Phospho-IkB-alpha (Ser32) Rabbit  (upper), or IkB-alpha Rabbit  (lower).

Catalog Number:MAB-94338
Conjugate:Unconjugated
Size:100 ul
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Ser32/Ser36. AA range:15-64
Reactivity:Hu, Ms, Rt, Mk
Applications:Western Blot: 1:500 -  1:2000. Immunohistochemistry: 1:100 - 1:300. Immunofluorescence: 1:200 - 1:1000. ELISA: 1:10000
Molecular Weight:40 kDa
Form:Liquid
Synonyms:NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3

Applications

Western Blot: 1:500 -  1:2000. Immunohistochemistry: 1:100 - 1:300. Immunofluorescence: 1:200 - 1:1000. ELISA: 1:10000

Immunogen

The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Ser32/Ser36. AA range:15-64

Target Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Synonyms:NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.

Buffer

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with TNF-alpha for 5 minutes., using Phospho-IkB-alpha (Ser32) Rabbit  (upper), or IkB-alpha Rabbit  (lower).

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