Immunological Sciences

Phospho KAT-5 (Ser 86) Rabbit Polyclonal Antibody

Western Blot analysis of Jurkat cells using Phospho-TIP60 (S86) Polyclonal Antibody

Western Blot analysis of Jurkat cells using Phospho-TIP60 (S86) Polyclonal Antibody

Catalog Number:ABP11224
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human TIP60 around the phosphorylation site of Ser86. AA range:52-101
Reactivity:Human;Mouse
Applications:Western Blot: 1:500-2000 Immunohistochemistry (paraffin-embedded tissues): 1:50-300 ELISA 2000-20000
Molecular Weight:65kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:KAT5; HTATIP; TIP60; Histone acetyltransferase KAT5; 60 kDa Tat-interactive protein; Tip60; Histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; Lysine acetyltransferase 5; cPLA(2)-interacting protein

Applications

Western Blot: 1:500-2000 Immunohistochemistry (paraffin-embedded tissues): 1:50-300 ELISA 2000-20000

Immunogen

The antiserum was produced against synthesized peptide derived from human TIP60 around the phosphorylation site of Ser86. AA range:52-101

Target Background

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants.

Synonyms:KAT5; HTATIP; TIP60; Histone acetyltransferase KAT5; 60 kDa Tat-interactive protein; Tip60; Histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; Lysine acetyltransferase 5; cPLA(2)-interacting protein

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of Jurkat cells using Phospho-TIP60 (S86) Polyclonal Antibody

Western blot analysis of lysates from Jurkat cells, using TIP60 (Phospho-Ser86) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIP60 (Phospho-Ser86) Antibody

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