Immunological Sciences

Phospho-MAPKAPK-2 (T334)

Western blot analysis of lysates from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) Antibody. The lane on the right is blocked with the phospho peptide

Western blot analysis of lysates from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) Antibody. The lane on the right is blocked with the phospho peptide

Catalog Number:ABP-0396
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:Synthesized peptide derived from human MAPKAPK-2 around the phosphorylation site of T334
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 ELISA: 1:20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Form:liquid

Applications

Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 ELISA: 1:20000

Immunogen

Synthesized peptide derived from human MAPKAPK-2 around the phosphorylation site of T334

Target Background

Phospho-MAPKAPK-2 (T334) polyclonal antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at T334.

Storage

Store at-20°C, and avoid repeat freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysates from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) Antibody. The lane on the right is blocked with the phospho peptide

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Thr334) Antibody. The picture on the right is blocked with the phospho peptide.

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