Immunological Sciences

Phospho-MYC-T58

Western blot analysis of extracts of 293T cells, using Phospho-MYC-T58 antibody at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s

Western blot analysis of extracts of 293T cells, using Phospho-MYC-T58 antibody at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s

Catalog Number:ABP-0080
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A phospho specific peptide corresponding to residues surrounding T58 of human MYC.
Reactivity:Hu
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100
Molecular Weight:62KDa
Purification:Affinity purification
Form:liquid
Synonyms:MRTL;MYCC;bHLHe39;c-Myc;MYC

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100

Immunogen

A phospho specific peptide corresponding to residues surrounding T58 of human MYC.

Target Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Synonyms:MRTL;MYCC;bHLHe39;c-Myc;MYC

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of 293T cells, using Phospho-MYC-T58 antibody at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s

Immunohistochemistry of paraffinembedded rat testis using P-MYC-T58 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded human lung cancer using PMYC- T58 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffinembedded mouse testis using P-MYC-T58 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using Phospho-MYC-T58 antibody. Blue: DAPI for nuclear staining.

No documents available.