Phospho-NF-KB p65 (Ser536) Rabbit Polyclonal Antibody

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Catalog Number:ABN00827
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser536. AA range:502-551
Reactivity:Human,Mouse,Rat,Monkey
Applications:WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,ELISA 1:5000-1:20000,IP 1:20-1:50
Molecular Weight:Calculated MW: 60 kDa; Observed MW: 60 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB

Applications

WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,ELISA 1:5000-1:20000,IP 1:20-1:50

Immunogen

The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser536. AA range:502-551

Target Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.

Synonyms:NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

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