Phospho-NF-κB2-S866/S870 Rabbit mAb
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-κB2-S866/S870 Rabbit mAb (AP1391) at 1:4000 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
| Catalog Number: | AB1391 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | ARC57764 |
| Immunogen: | A synthetic phosphorylated peptide around S866&S870 of human NF. |
| Reactivity: | Human,Mouse |
| Applications: | WB,1:1000 - 1:5000 IHC-P,1:50 - 1:200 |
| Molecular Weight: | 110kDa |
| Purification: | Affinity purification |
| Form: | liquid |
This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.
Applications
WB,1:1000 - 1:5000 IHC-P,1:50 - 1:200
Immunogen
A synthetic phosphorylated peptide around S866&S870 of human NF.
