Immunological Sciences

Phospho-NFκB-p65 (S311)

Western Blot analysis of various cells using Phospho-NFκB-p65 (S311) Polyclonal Antibody

Western Blot analysis of various cells using Phospho-NFκB-p65 (S311) Polyclonal Antibody

Catalog Number:APB-0445
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Poly
Immunogen:The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser311. AA range:278-327
Reactivity:Human;Mouse
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000.
Molecular Weight:65 kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:liquid
Synonyms:RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000.

Immunogen

The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser311. AA range:278-327

Target Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappaB moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, NFKB-P65, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, NFKB-P65. Four transcript variants encoding different isoforms have been found for this gene.

Synonyms:RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser311. AA range:278-327

Western Blot analysis of various cells using Phospho-NFκB-p65 (S311) Polyclonal Antibody

Western Blot analysis of 3T3 cells using Phospho-NFκB-p65 (S311) Polyclonal Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NF-kappaB p65 (Phospho-Ser311) Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Ser311) Antibody. The picture on the right is blocked with the phospho peptide.

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