Immunological Sciences

Phospho-NFκB-p65 (S468)

Western blot analysis of extracts from Hela cells, using Phospho-NFKB-p65-S468 antibody

Western blot analysis of extracts from Hela cells, using Phospho-NFKB-p65-S468 antibody

Catalog Number:ABP-0446
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A phospho specific peptide corresponding to residues surrounding S468 of h uman NFKB-P65
Reactivity:Hu, Ms, Rt
Applications:Western Blotting 1:1000 Immunofluorescence 1:100 - 1:200
Molecular Weight:"65 kDa "
Purification:Affinity purification
Form:liquid
Synonyms:p65; NFKB3; RELA

Applications

Western Blotting 1:1000 Immunofluorescence 1:100 - 1:200

Immunogen

A phospho specific peptide corresponding to residues surrounding S468 of h uman NFKB-P65

Target Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa¬B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, NFKB-P65, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, NFKB-P65. Four transcript variants encoding different isoforms have been found for this gene.

Synonyms:p65; NFKB3; RELA

Storage

Store at -20° C. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3, BSA.

Western blot analysis of extracts from Hela cells, using Phospho-NFKB-p65-S468 antibody

Immunofluorescence staining of methanol¬fixed HeLa cells, using Phospho-NFKB-p65 -S468 antibody.

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