Phospho-p27 Kip 1 (Ser10) Rabbit Monoclonal Antibody

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Catalog Number:MABN84871
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic phosphopeptide corresponding to residues surrounding Ser10 of human p27 KIP 1
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,IHC 1:50-1:100,IP 1:10-1:20
Molecular Weight:Calculated MW: 22 kDa; Observed MW: 27 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

Applications

WB 1:500-1:1000,IHC 1:50-1:100,IP 1:10-1:20

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser10 of human p27 KIP 1

Target Background

The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Synonyms:CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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