Immunological Sciences

Phospho-PI 3-kinase p85α (Try607) Rabbit Polyclonal Antibody

Western Blot analysis of various cells using Phospho-PI 3-kinase p85α (Tyr607) Polyclonal Antibody diluted at 1:1000

Western Blot analysis of various cells using Phospho-PI 3-kinase p85α (Tyr607) Polyclonal Antibody diluted at 1:1000

Catalog Number:ABP10906
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha around the phosphorylation site of Tyr607. AA range:573-622
Reactivity:Hu, Ms, Rt, Chicken
Applications:Western Blot: 1/500 - 1/2000 Immunofluorescence:: 1:50-200 Immunohistochemistry: 1/100 - 1/300.
Molecular Weight:80kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:liquid
Synonyms:PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

Applications

Western Blot: 1/500 - 1/2000 Immunofluorescence:: 1:50-200 Immunohistochemistry: 1/100 - 1/300.

Immunogen

The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha around the phosphorylation site of Tyr607. AA range:573-622

Target Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Synonyms:PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

Storage

Store at -20℃. Avoid freeze / thaw cycles

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using Phospho-PI 3-kinase p85α (Tyr607) Polyclonal Antibody diluted at 1:1000

Western Blot analysis of KB cells using Phospho-PI 3-kinase p85α (Tyr607) Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Siha cell. 1,primary Antibody was diluted at 1:100(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500(room temperature, 50min).

Immunofluorescence analysis of rat-heart tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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