Phospho-PKC (pan) (βII Ser660)

Western blot analysis of extracts of 293T cells, using Phospho-PKC (pan) (βII Ser660) antibody at 1:1000 dilution. 293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECLWest Pico Plus.  Exposure time: 90s.

Western blot analysis of extracts of 293T cells, using Phospho-PKC (pan) (βII Ser660) antibody at 1:1000 dilution. 293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECLWest Pico Plus. Exposure time: 90s.

Catalog Number:ABP-0495
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A phospho specific peptide corresponding to residues surrounding Ser660 of human PKC (pan) βII
Reactivity:Hu, Ms, Rt
Applications:WB 1:1000
Molecular Weight:95kDa
Purification:Affinity purification
Form:liquid

Applications

WB 1:1000

Immunogen

A phospho specific peptide corresponding to residues surrounding Ser660 of human PKC (pan) βII

Target Background

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Western blot analysis of extracts of 293T cells, using Phospho-PKC (pan) (βII Ser660) antibody at 1:1000 dilution. 293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECLWest Pico Plus. Exposure time: 90s.

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