Immunological Sciences

Phospho-Pyruvate Dehydrogenase E1-alpha subunit (DGR14223) Rabbit Monoclonal Antibody

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Catalog Number:MABN87115
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic phosphopeptide corresponding to residues surrounding Ser293 of human Pyruvate Dehydrogenase E1-alpha subunit
Reactivity:Human,Mouse,Rat
Applications:WB 1:1000-1:5000,IHC 1:100-1:200,IP 1:10-1:100
Molecular Weight:Calculated MW:43 kDa; Observed MW:43 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:PDHA; PDHAD; PHE1A; PDHCE1A

Applications

WB 1:1000-1:5000,IHC 1:100-1:200,IP 1:10-1:100

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser293 of human Pyruvate Dehydrogenase E1-alpha subunit

Target Background

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

Synonyms:PDHA; PDHAD; PHE1A; PDHCE1A

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

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