Phospho-Raf1 (Ser259) Rabbit Monoclonal antibody

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Catalog Number:MABN02515
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic phosphopeptide corresponding to residues surrounding Ser259 of human Raf1
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,IHC 1:50-1:100
Molecular Weight:Calculated MW: 73 kDa; Observed MW: 73 kDa
Purification:Affinity Purified
Form:Liquid
Synonyms:RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Applications

WB 1:500-1:1000,IHC 1:50-1:100

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser259 of human Raf1

Target Background

Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.

Synonyms:RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

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