Phospho-Rb (Ser780) Rabbit Monoclonal Antibody

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Catalog Number:MABN84879
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Rb
Reactivity:Human,Rat
Applications:WB 1:500-1:1000,IP 1:10-1:20
Molecular Weight:Calculated MW: 106 kDa; Observed MW: 110 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

Applications

WB 1:500-1:1000,IP 1:10-1:20

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Rb

Target Background

Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Synonyms:RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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