Phospho-Rb2 p130 (Thr986) Rabbit Monoclonal Antibody

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Catalog Number:MABN84838
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic phosphopeptide corresponding to residues surrounding Thr986 of human p130
Reactivity:Human
Applications:WB 1:500-1:1000,IP 1:10-1:20
Molecular Weight:Calculated MW: 128 kDa; Observed MW: 128 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:Rb2; P130

Applications

WB 1:500-1:1000,IP 1:10-1:20

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Thr986 of human p130

Target Background

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

Synonyms:Rb2; P130

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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