Phospho-Src (Tyr530) Rabbit Monoclonal Antibody

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Catalog Number:MABN02877
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic Phosphorylated peptide corresponding to residues target protein
Reactivity:Rat
Applications:WB 1:500-1:1000,IP 1:20-1:50
Molecular Weight:Calculated MW: 60 kDa; Observed MW: 60 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Applications

WB 1:500-1:1000,IP 1:20-1:50

Immunogen

A synthetic Phosphorylated peptide corresponding to residues target protein

Target Background

Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Synonyms:SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

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