Immunological Sciences

Phospho-Stat3 (Y705)

Western Blot analysis of our customer's samples using primary antibody diluted at 1:500, The picture was kindly provided by our customer.

Western Blot analysis of our customer's samples using primary antibody diluted at 1:500, The picture was kindly provided by our customer.

Catalog Number:ABP-4001
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:Synthesized peptide derived from human Stat3 around the phosphorylation site of Tyr705.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:200 Immunofluorescence: 1:100 Immunoprecipitation: 1-2,5 ug/mg lysate ELISA: 1:5000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:liquid

Applications

Western Blot: 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:200 Immunofluorescence: 1:100 Immunoprecipitation: 1-2,5 ug/mg lysate ELISA: 1:5000

Immunogen

Synthesized peptide derived from human Stat3 around the phosphorylation site of Tyr705.

Target Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.Phospho-Stat3 (Tyr705) Polyclonal Antibody detects endogenous levels of Stat3 protein only when phosphorylated at Tyr705

Storage

Store at –20°C. Do not aliquot the antibody.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of our customer's samples using primary antibody diluted at 1:500, The picture was kindly provided by our customer.

Western Blot analysis of various cells using Phospho-Stat3 (Tyr705) Polyclonal Antibody diluted at 1:1000

Immunofluorescence analysis of mouse-spleen tissue. A) Stat3 (phospho Tyr705) Polyclonal Antibody(red) was diluted at 1:100(4°C,overnight). B) Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).C) Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

IHC(P) on Human-lung-cancer tissue. Stat3 (phospho Tyr705) 1:200 (4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody at 1:200(RT 30min). Negative control was used by secondary antibody only.

IHC(P) Human-Appendix tissue. Stat3 (phospho Tyr705) 1:200 (4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). Secondary antibody was diluted at 1:200(RT 30min). Negative control was used by secondary antibody only.

IHC(P) Rat-lung tissue. Stat3 (phospho Tyr705) 1:200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). Secondary antibody was diluted at 1:200 (RT, 30min). Negative control was used by secondary antibody only.

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