Immunological Sciences

Phospho-STAT5A-Y694

Western blot analysis of extracts ofK562 cells, using Phospho-STAT5A-Y694 antibody at 1:2000dilution. K562 cells were treated byG-CSF (25ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA. Detection: ECL Exposure time: 90s

Western blot analysis of extracts ofK562 cells, using Phospho-STAT5A-Y694 antibody at 1:2000dilution. K562 cells were treated byG-CSF (25ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA. Detection: ECL Exposure time: 90s

Catalog Number:ABP-0138
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A phospho specific peptide corresponding to residues surrounding Tyr694 of human STAT5A
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:1000
Molecular Weight:105kDa
Purification:Affinity purification
Form:liquid

Applications

Western Blot: 1:1000

Immunogen

A phospho specific peptide corresponding to residues surrounding Tyr694 of human STAT5A

Target Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts ofK562 cells, using Phospho-STAT5A-Y694 antibody at 1:2000dilution. K562 cells were treated byG-CSF (25ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA. Detection: ECL Exposure time: 90s

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