Immunological Sciences

Phospho-TrkA (Tyr496) Rabbit Polyclonal Antibody

Western blot analysis of various lysates. Primary antibody was diluted at 1:1000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Western blot analysis of various lysates. Primary antibody was diluted at 1:1000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Catalog Number:ABP-11213
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Poly
Immunogen:Synthesized pospho peptide derived from human Trk A (Phospho-Tyr496)
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:100 - 1:500 ELISA: 1:5000 - 1:20000
Molecular Weight:180 kDa
Purification:Affinity Purified
Form:Liquid
Synonyms:NTRK1; MTC; TRK; TRKA

Applications

Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:100 - 1:500 ELISA: 1:5000 - 1:20000

Immunogen

Synthesized pospho peptide derived from human Trk A (Phospho-Tyr496)

Target Background

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

Synonyms:NTRK1; MTC; TRK; TRKA

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Western blot analysis of various lysates. Primary antibody was diluted at 1:1000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Immunofluorescence analysis of NIH/3T3 cells, using Phospho-TrkA (Tyr496) Antibody. The picture on the right is blocked with the phospho peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Phospho-TrkA (Tyr496) Antibody

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