Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody
Western blot analysis of TrkB using anti-TrkB antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkB antigen affinity purified monoclonal antibody at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TrkB at approximately 140 kDa.
| Catalog Number: | MAB-95050 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | EGA-14 |
| Immunogen: | A synthesized peptide derived from human Phospho-TrkB (Y817). |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunocytochemistry: 1:50-1:100 Immunofluorescence:1:50-1:100 Immunoprecipitation: 1:30 |
| Molecular Weight: | ∼140 kDa |
| Purification: | Affinity-chromatography |
| Form: | Liquid |
Applications
Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunocytochemistry: 1:50-1:100 Immunofluorescence:1:50-1:100 Immunoprecipitation: 1:30
Immunogen
A synthesized peptide derived from human Phospho-TrkB (Y817).
