Immunological Sciences

Phospho-YAP1-S127 pAb

Western blot analysis of extracts of NIH3T3 cells, using Phospho-YAP1-S127 antibody at 1:1000 dilution.NIH3T3 cells were treated by Sorbitol (0.2M) at 37℃ for 60 minutes after serum starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus Exposure time: 60s.

Western blot analysis of extracts of NIH3T3 cells, using Phospho-YAP1-S127 antibody at 1:1000 dilution.NIH3T3 cells were treated by Sorbitol (0.2M) at 37℃ for 60 minutes after serum starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus Exposure time: 60s.

Catalog Number:ABP-0489
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic phosphorylated peptide around S127 of human YAP1.
Reactivity:Hu, Ms,Rt
Applications:Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100
Molecular Weight:68kDa
Form:liquid
Synonyms:COB1;YAP;YAP2;YAP65;YKI;YAP1

Applications

Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100

Immunogen

A synthetic phosphorylated peptide around S127 of human YAP1.

Target Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms:COB1;YAP;YAP2;YAP65;YKI;YAP1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of NIH3T3 cells, using Phospho-YAP1-S127 antibody at 1:1000 dilution.NIH3T3 cells were treated by Sorbitol (0.2M) at 37℃ for 60 minutes after serum starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus Exposure time: 60s.

Immunohistochemistry of paraffin embedded human colon carcinoma using Phospho-YAP1-S127 Rabbit pAb at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Phospho-YAP1-S127 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Phospho-YAP1-S127 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review for Phospho-YAP1-S127 Rabbit pAb: Experiment Type: Western blot(WB) Sample: THP1

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