Immunological Sciences

Phospho-ZAP-70 (Y319) Rabbit Polyclonal Antibody

Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr319) Antibody. The lane on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr319) Antibody. The lane on the right is blocked with the phospho peptide.

Catalog Number:ABP11202
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Poly
Immunogen:The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr319. AA range:286-335
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100- 1:300 Immunofluorescence: 1:200 - 1:1000 ELISA: 1:20000
Molecular Weight:70kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:liquid
Synonyms:ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100- 1:300 Immunofluorescence: 1:200 - 1:1000 ELISA: 1:20000

Immunogen

The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr319. AA range:286-335

Target Background

Zeta chain of T cell receptor associated protein kinase 70(ZAP70) Homo sapiens This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Synonyms:ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr319) Antibody. The lane on the right is blocked with the phospho peptide.

<span dir="ltr" role="presentation">Immunohistochemical analysis of paraffin-embedded human </span><span dir="ltr" role="presentation">small intestinal carcinoma tissue. 1,primary Antibody was diluted </span><span dir="ltr" role="presentation">at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for </span><span dir="ltr" role="presentation">antigen retrieval(&gt;98°C,20min). 3,Secondary antibody was </span><span dir="ltr" role="presentation">diluted at 1:200</span>

Immunofluorescence analysis of Jurkat cells using Phospho-ZAP70-Y319 Rabbit pAb at dilution of 1:100 (40x lens).Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.

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