Immunological Sciences

PI 3-kinase p85α Rabbit Polyclonal Antibody

Western Blot analysis of various cells using PI 3-kinase p85α Polyclonal Antibody diluted at 1:1000

Western Blot analysis of various cells using PI 3-kinase p85α Polyclonal Antibody diluted at 1:1000

Catalog Number:AB-E12363
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha. AA range:573-622
Reactivity:Human;Mouse;Rat
Applications:Western Blot:   1:500/1:2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000.
Molecular Weight:85kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

Applications

Western Blot:   1:500/1:2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000.

Immunogen

The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha. AA range:573-622

Target Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Synonyms:PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using PI 3-kinase p85α Polyclonal Antibody diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human placenta. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PI3-kinase p85-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using PI3-kinase p85-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

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