Immunological Sciences

PKC alpha Rabbit Polyclonal antibody

Western Blot analysis of NIH-3T3 cells using PKC α Polyclonal Antibody

Western Blot analysis of NIH-3T3 cells using PKC α Polyclonal Antibody

Catalog Number:AB-90243
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human PKC alpha. AA range:606-655
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300
Molecular Weight:76kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha

Applications

Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300

Immunogen

The antiserum was produced against synthesized peptide derived from human PKC alpha. AA range:606-655

Target Background

diacylglycerol. PKC family members phosphorylate a vwide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Synonyms:PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of NIH-3T3 cells using PKC α Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human Right liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Right liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature.

Immunohistochemical analysis of paraffin-embedded Human Right liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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