Immunological Sciences

PKC zeta Rabbit Polyclonal Antibody

Western Blot analysis of various cells using PKC ζ Polyclonal Antibody diluted at 1:2000

Western Blot analysis of various cells using PKC ζ Polyclonal Antibody diluted at 1:2000

Catalog Number:AB-84799
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human PKC zeta. AA range:526-575
Reactivity:Human;Mouse;Rat;Monkey
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000.
Molecular Weight:80kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000.

Immunogen

The antiserum was produced against synthesized peptide derived from human PKC zeta. AA range:526-575

Target Background

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemicalproperties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms:PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using PKC ζ Polyclonal Antibody diluted at 1:2000

Western Blot analysis of COS7 cells using PKC ζ Polyclonal Antibody diluted at 1:2000

Immunofluorescence analysis of NIH/3T3 cells, using PKC zeta Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PKC zeta Antibody. The picture on the right is blocked with the synthesized peptide.

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