Immunological Sciences

PLA2G4A / cPLA2 Rabbit Polyclonal Antibody

Western blot analysis of lysates from HeLa cells, treated with TNF-a 20ng/ml 30', using c-PLA2 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, treated with TNF-a 20ng/ml 30', using c-PLA2 Antibody. The lane on the right is blocked with the synthesized peptide.

Catalog Number:AB-81803
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human c-PLA2. AA range:471-520
Reactivity:Human,Mouse,Rat
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/200 - 1/1000
Molecular Weight:110 kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/200 - 1/1000

Immunogen

The antiserum was produced against synthesized peptide derived from human c-PLA2. AA range:471-520

Target Background

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants.

Synonyms:PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysates from HeLa cells, treated with TNF-a 20ng/ml 30', using c-PLA2 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Hela cell. 1,cPLA2 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488  was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Immunofluorescence analysis of HUVEC cells, using c-PLA2 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using c-PLA2 Antibody. The picture on the right is blocked with the synthesized peptide.

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