PLK1 Rabbit Polyclonal Antibody

Western Blot analysis of extracts from NIH-3T3 cells, using Plk1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Western Blot analysis of extracts from NIH-3T3 cells, using Plk1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Catalog Number:AB-83900
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from Plk1 . at AA range: 80-160
Reactivity:Human;Mouse;Rat
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000
Molecular Weight:68kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000

Immunogen

Synthesized peptide derived from Plk1 . at AA range: 80-160

Target Background

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.

Synonyms:PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of extracts from NIH-3T3 cells, using Plk1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Immunofluorescence analysis of A549 cells, using PLK1 Antibody. The picture on the right is blocked with the synthesized peptide.

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