PRAS40 Rabbit Monoclonal Antibody

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Catalog Number:MABN03162
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic peptide of human PRAS40
Reactivity:Human,Rat
Applications:WB 1:500-1:1000,IP 1:20-1:50
Molecular Weight:Calculated MW: 27 kDa; Observed MW: 40 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate

Applications

WB 1:500-1:1000,IP 1:20-1:50

Immunogen

A synthetic peptide of human PRAS40

Target Background

PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.

Synonyms:AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

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