PSD95 Rabbit Polyclonal Antibody

Western blot analysis of various lysates using PSD95 Rabbit pAb  at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECLWest Pico Plus.  
Exposure time: 180s.

Western blot analysis of various lysates using PSD95 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECLWest Pico Plus. Exposure time: 180s.

Catalog Number:AB6194
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 420-510 of human PSD95.
Reactivity:Human,Mouse,Rat
Applications:Western Blot: 1:100 - 1:500 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunohistochemistry: 1:50-1:200
Molecular Weight:95kDa
Purification:Affinity purification
Form:liquid
Synonyms:MRD62; PSD95; SAP90; SAP-90

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Applications

Western Blot: 1:100 - 1:500 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunohistochemistry: 1:50-1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 420-510 of human PSD95.

Target Background

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms:MRD62; PSD95; SAP90; SAP-90

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.09% Sodium azide,50% glycerol,pH7.3.

Western blot analysis of various lysates using PSD95 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECLWest Pico Plus. Exposure time: 180s.

Immunofluorescence analysis of SH-SY5Y cells using PSD95 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

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