Immunological Sciences

Purified Anti-Human CD40 Antibody Azide free

Human peripheral blood lymphocytes were stained with 0.2 μg Purified Anti-Human CD40 Antibody, Functional Grade (Right) and 0.2 μg Mouse IgG1, κ Isotype Control (Left), followed by 647-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD19 PE-conjugated Monoclonal Antibody.

Human peripheral blood lymphocytes were stained with 0.2 μg Purified Anti-Human CD40 Antibody, Functional Grade (Right) and 0.2 μg Mouse IgG1, κ Isotype Control (Left), followed by 647-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD19 PE-conjugated Monoclonal Antibody.

Catalog Number:MAB11630
Conjugate:Unconjugated
Size:100 ug
Host:Mouse
Isotype:Mouse IgG1, κ
Clone:HI40a
Immunogen:Recombinant Human CD40 protein
Reactivity:Human
Applications:FCM ≤ 0.2 µg per million cells in 100 µL volume; Stim Reported in the literature
Molecular Weight:48 kD
Purification:>98%, Protein A/G purified
Form:Liquid
Synonyms:BP50
Download Datasheet

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

Applications

FCM ≤ 0.2 µg per million cells in 100 µL volume; Stim Reported in the literature

Immunogen

Recombinant Human CD40 protein

Target Background

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

Synonyms:BP50

Storage

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Human peripheral blood lymphocytes were stained with 0.2 μg Purified Anti-Human CD40 Antibody, Functional Grade (Right) and 0.2 μg Mouse IgG1, κ Isotype Control (Left), followed by 647-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD19 PE-conjugated Monoclonal Antibody.

Human peripheral blood lymphocytes were stained with 0.2 μg Purified Anti-Human CD40 Antibody, Functional Grade (Right) and 0.2 μg Mouse IgG1, κ Isotype Control (Left), followed by Brigthfluor 647- conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD19 PE conjugated Monoclonal Antibody.

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