Immunological Sciences

Pyruvate Dehydrogenase E1 α Rabbit Monoclonal Antibody

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Catalog Number:MABN21508
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG,Kappa
Clone:Monoclonal
Immunogen:A synthetic peptide corresponding to target protein
Reactivity:Human,Mouse,Rat,
Applications:WB 1:1000-1:5000,IHC 1:200-1:1000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200
Molecular Weight:CalcuLated MW:43kD;Observed MW:43kD
Purification:Protein A
Form:Liquid
Synonyms:PDHA1;PHE1A;Pyruvate dehydrogenase E1 component subunit alpha;somatic form, mitochondrial;PDHE1-A type I

Applications

WB 1:1000-1:5000,IHC 1:200-1:1000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200

Immunogen

A synthetic peptide corresponding to target protein

Target Background

Cell localization:Mitochondrion matrix.The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010],

Synonyms:PDHA1;PHE1A;Pyruvate dehydrogenase E1 component subunit alpha;somatic form, mitochondrial;PDHE1-A type I

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

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