RAB5A Rabbit Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using RAB5AA Rabbit mAb at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico plus. 
Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using RAB5AA Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico plus. Exposure time: 30s.

Catalog Number:MAB-94769
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:ARC0297
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 116-215 of human RAB5AA.
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:2000
Molecular Weight:25kDa
Purification:Affinity purification
Form:Liquid
Synonyms:RAB5

Applications

Western Blot: 1:500 - 1:2000

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 116-215 of human RAB5AA.

Target Background

Enables GDP binding activity; GTP binding activity; and GTPase activity. Involved in several processes, including amyloid-beta clearance by transcytosis; early endosome to late endosome transport; and regulation of exocytosis. Located in several cellular components, including cytoplasmic side of early endosome membrane; nucleoplasm; and terminal bouton.

Synonyms:RAB5

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using RAB5AA Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico plus. Exposure time: 30s.

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