Immunological Sciences

Recombinant Anti-AKT antibody (Rabbit mAb)

WB analysis of AKT . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of AKT . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

Catalog Number:ABS15689
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:KLH conjugated Synthetic peptide corresponding to Human AKT
Reactivity:Human, Mouse, Rat
Applications:IHC/IF<br/>1: 500-1: 1000
Molecular Weight:56 kDa / 60 kDa
Purification:Affinity purification
Form:Liquid

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Applications

IHC/IF<br/>1: 500-1: 1000

Immunogen

KLH conjugated Synthetic peptide corresponding to Human AKT

Target Background

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Storage

Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

WB analysis of AKT . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of AKT . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4℃ overnight. Secondary antibody :HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

IHC analysis of AKT. Sample: Human cervical carcinoma, 4% PFA 12-24h. Antigen retrieval: Citrate buffer , pressure cooker 2min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IHC analysis of AKT. Sample: Human gastric carcinoma, 4% PFA 12-24h. Antigen retrieval: Citrate buffer , pressure cooker 2min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IHC analysis of AKT. Sample: Mouse colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer , pressure cooker 2min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IHC analysis of AKT. Sample: Mouse subcutaneous nude mouse model of 4T1 cell, 4% PFA 12-24h. Antigen retrieval: Citrate buffer , pressure cooker 2min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IHC analysis of AKT. Sample: Mouse testis, 4% PFA 12-24h. Antigen retrieval: Citrate buffer , pressure cooker 2min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IHC analysis of AKT. Sample: Rat testis, 4% PFA 12-24h. Antigen retrieval: Citrate buffer , pressure cooker 2min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

IHC analysis of AKT. Sample: Rat colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer , pressure cooker 2min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

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