Immunological Sciences

Recombinant Anti-beta Actin Rabbit Monoclonal Antibody

WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

Catalog Number:MABS15003
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:13E5
Immunogen:KLH conjugated synthetic peptide corresponding to Mouse beta-Actin
Reactivity:Species, Human, Mouse, Rat, Pg, Ch, Bv, Sh, Gt, Mk, Rb, Ta, Frog, Human, Mouse, Rat
Applications:WB 1: 3000-1: 5000<br/>IHC/IF 1: 500-1: 1000<br/>ICC/IF 1: 300-1: 1000
Molecular Weight:42 kDa / 42 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta CytoskeletalActin, β-Actin
Download Datasheet

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Applications

WB 1: 3000-1: 5000<br/>IHC/IF 1: 500-1: 1000<br/>ICC/IF 1: 300-1: 1000

Immunogen

KLH conjugated synthetic peptide corresponding to Mouse beta-Actin

Target Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Synonyms:ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta CytoskeletalActin, β-Actin

Storage

Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

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