Recombinant Anti-COX2/Cyclooxygenase 2 antibody (Rabbit mAb)

WB analysis of COX2/Cyclooxygenase 2. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

WB analysis of COX2/Cyclooxygenase 2. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

Catalog Number:ABS155672
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG,κ
Clone:2COX
Immunogen:Recombinant protein corresponding to Human COX2
Reactivity:Human, Mouse, Rat
Applications:WB<br/>1: 500-1: 1500 IHC/IF 1: 100-1: 300
Molecular Weight:74 kDA
Purification:Affinity purification
Form:Liquid

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.

Applications

WB<br/>1: 500-1: 1500 IHC/IF 1: 100-1: 300

Immunogen

Recombinant protein corresponding to Human COX2

Target Background

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

WB analysis of COX2/Cyclooxygenase 2. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

IHC analysis of COX2/Cyclooxygenase 2. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: TE buffer , pressure cooker 2 min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG , Ready to use, RT, 20min.

IHC analysis of COX2/Cyclooxygenase 2. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: TE buffer , pressure cooker 2 min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG , Ready to use, RT, 20min.

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