Recombinant Anti-FOXO1 antibody

IHC analysis of FOXO1 antibody
Sample: Mouse heart, 4% PFA 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0), 100℃ 25min.
Primary antibody: 1: 10000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use, RT, 20min.

IHC analysis of FOXO1 antibody Sample: Mouse heart, 4% PFA 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0), 100℃ 25min. Primary antibody: 1: 10000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use, RT, 20min.

Catalog Number:ABS154935
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:KLH conjugated Synthetic peptide corresponding to Human FOXO1
Reactivity:Human,Mouse,Rat
Applications:Western Blot: 1: 1000-1: 2000 Immunohistochemistry: 1: 10000-1: 20000 Immunofluorescence: 1: 10000-1: 20000
Molecular Weight:70 kDa / 100 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:FOXO1, FKH1, FKHR, FOXO1A, forkhead box O1

FOXO1 is a transcription factor that plays important roles in regulation ofgluconeogenesis and glycogenolysis by insulin signaling, and is also central to the decision for a preadipocyte to commit to adipogenesis. It is primarily regulated through phosphorylation on multiple residues; its transcriptional activity is dependent on its phosphorylation state.

Applications

Western Blot: 1: 1000-1: 2000 Immunohistochemistry: 1: 10000-1: 20000 Immunofluorescence: 1: 10000-1: 20000

Immunogen

KLH conjugated Synthetic peptide corresponding to Human FOXO1

Target Background

FOXO1 is a transcription factor that plays important roles in regulation ofgluconeogenesis and glycogenolysis by insulin signaling, and is also central to the decision for a preadipocyte to commit to adipogenesis. It is primarily regulated through phosphorylation on multiple residues; its transcriptional activity is dependent on its phosphorylation state.

Synonyms:FOXO1, FKH1, FKHR, FOXO1A, forkhead box O1

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

IHC analysis of FOXO1 antibody Sample: Mouse heart, 4% PFA 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0), 100℃ 25min. Primary antibody: 1: 10000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use, RT, 20min.

IF analysis of FOXO1 antibody red. Sample: Rat testis, 4% PFA 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) 100℃ 25min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1: 10000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) , 1: 300, RT, 1h.

Western blot analysis of lysates from HeLa cells, treated with EGF+Serum, using FOXO1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using FOXO1 Antibody. The picture on the right is blocked with the synthesized peptide.

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