Recombinant Anti-GAPDH antibody Rabbit Monoclonal Antibody
WB analysis of GAPDH.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 5000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
| Catalog Number: | ABS15004 |
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG, κ |
| Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse GAPDH |
| Reactivity: | Species, Human, Mouse, Rat, Pg, Ch, Bv, Sh, Gt, Mk, Rb, Ta, Frog, Human, Mouse, Rat |
| Applications: | Applications Dilution<br/>WB 1: 3000-1: 5000<br/>IHC/IF 1: 500-1: 2000<br/>ICC/IF 1: 500-1: 1000 |
| Molecular Weight: | 36 kDa / 36 kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.
Applications
Applications Dilution<br/>WB 1: 3000-1: 5000<br/>IHC/IF 1: 500-1: 2000<br/>ICC/IF 1: 500-1: 1000
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse GAPDH
