Recombinant Anti-Phospho-AKT (S473) antibody (Rabbit mAb)
WB analysis of Phospho-AKT(S473). Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG 1: 3000, RT, 1h.
| Catalog Number: | MABS150002 |
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | D702 |
| Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human AKT(S473) |
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB<br/>1: 1000-1: 2000 |
| Molecular Weight: | 60 kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT |
An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK.These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr.Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation.While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Images
Applications
WB<br/>1: 1000-1: 2000
Immunogen
KLH conjugated Synthetic phosphopeptide corresponding to Human AKT(S473)