Recombinant Anti-Vinculin Mouse Monoclonal Antibody
WB analysis of Vinculin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 5000, RT, 1h.
| Catalog Number: | MABS150068 |
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG1 |
| Clone: | VC12 |
| Immunogen: | Recombinant protein corresponding to Human Vinculin |
| Reactivity: | Species, Human, Mouse, Rat |
| Applications: | Applications Dilution<br/>WB 1: 1000-1: 2000<br/>IHC/IF 1: 1000-1: 2000 |
| Molecular Weight: | 124 kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | Metavinculin, Vcl, CMD1W, CMH15, MVCL, MV, VINC |
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion.
Applications
Applications Dilution<br/>WB 1: 1000-1: 2000<br/>IHC/IF 1: 1000-1: 2000
Immunogen
Recombinant protein corresponding to Human Vinculin
