RNASEH2A Rabbit Polyclonal Antibody
Western blot analysis of various lysates using RNASEH2A Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.
| Catalog Number: | AB15132 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Reactivity: | Human,Rat |
| Applications: | WB 1:500 - 1:2000; IF/ICC 1:50 - 1:200; ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 33kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | AGS4; JUNB; RNHL; RNHIA; THSD8; RNASEHI; RNASEH2A |
The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.
Applications
WB 1:500 - 1:2000; IF/ICC 1:50 - 1:200; ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
